Abstract:【Abstract】 Objective To explore the effect of sCD14 on premature rupture of membrane(PROM) and perinatal infection in peripheral blood. Methods From April 2015 to December 2016, 50 cases of PROM primiparas were selected as research group, 50 cases of normal primiparas were selected as control group. Elisa method was used to detect the sCD14 level in the blood of the mother, the umbilicus, and the perinatal infant. According to the time of PROM and the infection of the perinatal infants to group, the sCD14 levels in mother, umbilicus and peripheral infant were observed. Results The level of sCD14 in mother, umbilicus and peripheral infant of the research group were significantly higher than that in the control group (P<0.01). In research group, the level of sCD14 in mother, umbilicus and peripheral infant were positively correlated. The longer the PROM time, the higher the sCD14 level (P<0.01). The levels of sCD14 in mother, umbilicus and peripheral infant were significantly higher in peripheral infant infection group than the noninfected group (P<0.01). Conclusion The level of sCD14 is related to the development of PROM and perinatal infant infection, which has certain guiding value for clinical diagnosis.