【Abstract】 Objective To measure the roles of syringin (SYR) on hypoxia/reoxygenation (H/R)treated cardiomyocytes and possible mechanims. Methods Cultured cardiomyocytes were assigned into control group, H/R group and H/R+SYR group. Three hours of hypoxia and 3h of reoxygenation were performed to cause H/R injury. Cell viability, LDH release, apoptosis, ROS, inflammation and molecular alternations were systematically detected. Moreover, the specific AMPK inhibitor (Compound c) was added in mechanistic measurement. Results In comparison with the control group, H/R resulted in the reduced cell viability and increased LDH release, followed by the worsened ROS, apoptosis and inflammation. However, above parameters induced by H/R injury were significantly attenuated by SYR treatment. Mechanistic experiment showed SYR administration enhanced the level of pAMPK, and blocking AMPK with the specific inhibitor blunted H/Rrepressing effects of SYR. Conclusion SYR attenuates myocardial H/R injury in part via a AMPKdependent manner.