【Abstract】 Objective To analyze the effects of cryoablation combined with granulocytemacrophage colonystimulating factor (GMCSF) on angiogenesis and cell proliferation in renal carcinoma mice. Methods 40 BALB mice with the health level at SPF were taken. The renal carcinoma mouse model was established with renal carcinoma cell line 7860. The mice with renal carcinoma successful establishing the model were randomly divided into A, B, C, and D group. Group A did not execute any treatment, group B was given GMCSF, group C was given cryoablation treatment and group D was given cryoablation combined with GMCSF. The basic survival status of the mice was recorded. The tumor changes of the mice were observed. The line chart of tumor size was drawn. The HUVEC tubule formation experiment and MTT were respectively used to detect angiogenesis and cell proliferation in each group. Results After the mice were inoculated with renal carcinoma cell line 7860, there was no obvious abnormal eating phenomenon with mild energielos, poor appetite. The inoculation site gradually expanded with time. The epidermis of the inoculation site showed no ulceration and bleeding. At 2 weeks after inoculation, there was no death mouse. HE staining confirmed that the mouse model with renal carcinoma was successfully established. In the group A, the tumor gradually increased at 1, 2, and 3 weeks after treatment. The tumor in the group A also continued to increase. The increase degree of group A was larger than that of group B. The tumor became small in group C and group D at 2 weeks after treatment. There was statistical significance in the difference of tumor size in the 4 groups at different time points, the tumor size in groups C and D at 2 and 4 weeks after treatment was significantly smaller than that in group A and B (P＜005). The length and number of lumen formation in group B, C and D were significantly lower than those in group A (P＜005), while the length and number of lumen formation in group D were significantly lower than those in group B and C (P＜005). The difference in cell proliferation inhibition rates of the mice in group B, C and D at 24h, 48h, 72h, 96h and 128h was statistically significant the (P＜005). The inhibition rate of cell proliferation was the highest at 72h after treatment, it decreased at 96h and 128h, the inhibition rate of cell proliferation in group D was significantly higher than that in group B and C at any time (P＜005). Conclusion Cryoablation combined with GMCSF therapy can significantly inhibit angiogenesis and cell proliferation in renal carcinoma mice and reduce tumor volume. The safety is good.