【Abstract】 Objective To explore the clinical value of combined detection of mALB, UCr, αMG, β2MG, and NAG in the early diagnosis of renal injury in diabetic nephropathy. METHODS 500 patients with diabetic nephropathy who were treated in our hospital from June 2016 to June 2018 were randomly selected as the study group and 500 healthy people examined in our hospital were selected as the control group. The urine in the two groups was measured. The contents of mALB, UCr, αMG, β2MG, and NAG were compared between the two groups. Results The ratios of UCr/Cr, MALB/Cr, β 2 MG/Cr, and NAG/C in the study group were significantly higher than those in the control group (P＜005). The individual indicators of mALB, UCr, β2MG, and NAG were used. The positive rate was significantly lower than that of the twoway test using mALB+UCr and β2MG+NAG (P＜005), and the combined test of mALB+UCr+β2MG+NAG+αMG was used. The positive rate was significantly higher than the positive rate of the mALB, UCr, β2MG, NAG single index and the positive rate of the twoway test of mALB+UCr and β2MG+NAG (P＜005). The mALB+ was used. The positive rate of UCr+β2MG+NAG+αMG combined test was significantly higher than that of serum creatinine (P＜005). mALB+UCr+β2MG+NAG+αMG was used. The positive rate of the combined test of the MG five indicators was significantly higher than that of the urea nitrogen (P＜005), and the combined test of the five indicators of mALB+UCr+β2MG+NAG+αMG was positive. The rate was significantly higher than that of endogenous creatinine (P＜005). Conclusion Combined detection of mALB, UCr, αMG, β2MG, and NAG can sensitively measure the physical changes in patients with diabetic nephropathy, so that patients can be treated as soon as possible, and the damage of renal tubules is longer than that of glomerular injury. Early, it is worth further promotion and application in clinical practice.