Objective To explore the association between Xray repair cross complementing group 1 (XRCC1) gene rs25487 locus polymorphisms in sperm DNA of oligoasthenotspermia. Methods 182 oligoasthenotspermia patients and 73 healthy male were recruited in this study. Their semens were collected once semen routine analysis had done. Then, we use polymerase chain reactionrestriction fragment length polymorphism (PCRRFLP) method to genotype the polymorphism of XRCC1 gene rs25487 locos. Results Compare with the healthy control group, the sperm concentration and motility were significantly decreased and the liquefaction were significantly prolonged in the oligozoospermia or azoospermia patients. AA genotype, compare with GG genotype, demonstrated a significantly increased risk for oligozoospermia or azoospermia patients (OR=7.84; 95%CI=1.019~60.365). Conclusion Our results demonstrated that AA genotypes in XRCC1 gene rs25478 locus may be a risk factor for oligozoospermia or azoospermia patients.