Objective To investigate the difference of anaerobic glycolysis ability of human erythrocytes suspended in vitro under different levels of hemoglobin (Hb). Methods According to the Hb level and living area, volunteers were divided into A group (at an altitude of more than 3500m, Hb＞180g/L) (10 cases), B group (at an altitude of more than or equal to 3500m, 160g/L≥Hb≥120g/L) (10 cases) and C group (at an altitude＜2500m, 160g/L≥Hb≥120g/L) (10 cases). 200ml whole blood collected from each volunteer of 3 different groups was centrifuged and the plasma was removed. Then the packed red blood cells were suspended in 50ml additive solution MAP and divided into four separate bags (50ml each) averagely, which were sampled aseptically and evaluated respectively at different periods of storage.Results In the 3 groups, the suspended red blood cells were tested on the first, seventh, twentyirst and 35 day. There was no statistical difference of the LDH ,ATP and glucose concentrations between the 3 groups (P＞0.05). Compared with the C group, ATP in A group was lower than that in group C on the seventh day (P＜0.05).Conclusion The 3 groups of suspended red blood cells in the preservation in vitro, on a number of indicators of erythrocyte glycolysis has no significant difference. The anaerobic metabolism of red blood cells in high altitude polycythemia is similar to that in normal population in plain.