Objective To observe the effect of MyD88 siRNA on the expression of MyD88 protein in dendritic cells.Methods The recombinant MyD88 RNAi fragment was ligated into plasmid vector and transformed into chemically competent Escherichia coli, then subjected to colony PCR identification, positive cloning and plasmid extraction. HEK293 cells were transfected and the recombinant adenovirus was transfected into two groups. The effect of MyD88siRNA on the expression of MyD88 protein in dendritic cells was detected by Western blot. The effect of MyD88 siRNA on the expression of MyD88 protein in dendritic cells was detected by Western blot. Results MyD88 siRNA could inhibit the expression of MyD88 protein in mucaca mulatta dendritic cells, and the expression of MyD88 protein was inhibited by Western blot. The positive clones were identified by PCR and sequenced. The dendritic cells were transfected by adenovirus vector. Western blot showed that the expression of MyD88 protein in the interference group was decreased.Conclusion The construction of mucaca mulatta MyD88 gene silencing siRNA adenovirus vector, MyD88siRNA in dendritic cells could inhibit the expression of MyD88 protein.