【Abstract】 Objective The aim of this study was to assay phenotype and genotype of vancomycin resistant enterococci(VRE) colonization strains that had been isolated from inpatient in hematology department and infectious disease department, investigate the features of VRE species constitution, resistant phenotypes and genotypes and provide evidences for clinical diagnosis, rational use of antimicrobials in VRE infections.Methods 170 rectal swabs were collected between January 2016 and December 2016 in pediatric department of Central Hospital of Zibo. VRE colonization was screened by inoculation and incubation. Minimum inhibitory concentrations of vancomycin, teicoplanin, linezolid, penicillin, gentamicin, ciprofloxacin, minocycline and ampicillin against VRE were detected by 2fold agar dilution method. The PCR amplification fragments were identified from their sizes in ethidium bromidestained 2% agarose gels and sequencing of amplicated gene fragments. Results 21 (124%) VRE strains were recovered from 170 rectal swabs. The antimicrobials resistant rates of VRE strains to the most of the determinated antimicrobials in this research was more than 50%,inclouding ciprofloxacin(905%), penicillin（81%）, ampicillin（762%）, gentamicin（HLAR 619%）. The sensitive rate to linezolid was 100% and sensitive rate to minocycline was 952%. 15 out of 21 strains of VRE were Enterococcus faecium 667%（14/21）. Genotypes of 21 strains of VRE were detected by PCR. That consisted of 18 strains of vanA. The patients with VRE colonization were followed up, nobody was found with VRE infection.Conclusion In our study, the most common strain of VRE was Enterococcus faecium, account for 667%. In vitro drug susceptibility tests suggested that VRE strains resistant to most antimicrobial agents, but still sensitive to linezolid rate and minocycline with sensitive rates＞90%, which can be candidates for chemotherapy for VRE infections. vanA was the most common genotype in the study of VRE strains. The more attentions should be paid to the disagreement between phenotype and genotype in clinical monitoring of VRE infections.