【Abstract】 Objective To detect the changes of anterior gradient2 (AGR2) level before and after operation in tissue and serum of patients with cervical intraepithelial neoplasia (CIN) and cervical cancer, and to explore its clinical significance. Methods The serum and tissue of patients with cervical cancer (n=30) and patients with CIN (n=20) before and after operation were collected. Serum sample from 20 health women and cervix tissue samples of 20 patients undertaken total hysterectomy due to myoma were collected for the control group. AGR2 levels were detected by enzymelinked immunosorbent assay (ELISA). Results The levels of AGR2 in preoperative serum and tissues of cervical cancer were significantly higher than that of CIN and normal control group (all P＜001). The levels of serum AGR2 after operation in the cervical cancer and CIN patients were significantly lower than that of preoperative (all P＜001). The AGR2 levels of serum and tissue of cervical cancer patients were significantly different for the tumor size, FIGO stages and lymph node involvement (P＜001 or 005). Pearson analysis showed that serum AGR2 levels before operation positively correlated to that after operation in the patients with CIN and cervical cancer (r=0747,P=0000). The levels of serum AGR2 before and after operation positively correlated to that in cervical tissue (r=0789,P=0000;r=0700,P=0000). Conclusion The levels of AGR2 in serum and tissue of cervical cancer increased obviously, which was closely related to tumor size, FIGO stages and lymph node involvement. It is valuable to dectect the level of AGR2 for judging the surgical curative effect of patients with cervical cancer.