【Abstract】 Objective To investigate the culture of tumorderived vascular endothelial cells (TdVECs) and identify their CD105 expression and targeting ability. Methods Enzyme digestion was used to isolate human umbilical vein endothelial cells (HUVECs). CD34 immunofluorescence staining was used to identify HUVECs. In order to acquire the characteristics of TdVECs, VECs were incubated with breast cancer cells in Millicell cell culture. The cell characteristics, molecular expression and targeted binding ability of TdVECs were detected by polymerase chain reaction (PCR), CD105 immunofluorescence and CD105targeted nanoparticles. Results HUVECs were successfully isolated. The expression of Tem1 and Tem8 in VECs was significantly increased after induction with breast cancer cells. Incubated VECs were positive for CD105 immunofluorescence staining, and the fluorescence intensity of induced VECs was stronger than that of noninduced VECs. After coincubation for 24 hours, at iron concentrations of 0, 05, 1, 2, 5, 10ug/mL, the labeling ratios were, respectively, 0%, (2535±423)%, (5807±412)%, (8668±342)%, 100% and 100% for induced VECs, and 0%, (1058±262)%, (1631±432)%, (4633±342)%, (7762±413)%, 100% for noninduced VECs. Conclusion Tumor cell coculture method can induce VECs to differentiate into TdVECs and is a reliable method to obtain TdVECs for CD105 molecular targeting studies.