【Abstract】 Objective To explore the injectable chitosan glycerophosphate (chitosan/glycerol phosphate disodium salt beta, C/GP) gel load of platelet rich plasma (plateletrich plasma, PRP) and bone marrow stromal stem cells (bone marrow mesenchymal stem (cells, BMSCs) and promote periodontal regeneration effect. Methods 30 second degree root bifurcation defect mode was made at premolar and first molar area of 3 healthy male dogs. 30 premolars were randomly into A group (C/GP), B group (C/GP+PRP), C group (C/GP+BMSCs), D group (C/GP+PRP+BMSCs) and control group. Intratoperatively, all periodontal defect flap suture, and injected gel in A group, B group, C group and D group. The control group was not treated with. Tissue samples were collected at 8 weeks after operation. Xray and histological observation and evaluation were carried out. Results The periodontal tissue regeneration of B group, C group and D group were obvious. The periodontal tissue regeneration of A group and control group were few.Conclusion C/GP loaded PRP and BMSCs can promote the regeneration of periodontal tissue.