YAP及P-YAP在RAW264.7细胞脓毒症模型中的表达
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泸州市科技计划项目(2015LZCYD-S05(11/12)


The research of YAP and P-YAP in sepsis model of raw264.7 cell
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    【摘要】 目的 探讨YAP及PYAP在RAW2647细胞脓毒症模型中的表达。方法 将对数生长期的RAW2647细胞用内毒素(LPS)刺激RAW2647细胞构建细胞脓毒症模型为实验组,未处理者为正常组。运用免疫荧光对YAP及PYAP进行定位,Western blot检测YAP及P-YAP的表达情况。结果 实验组YAP蛋白表达于胞核中,而P-YAP表达于胞质中。实验组YAP蛋白表达明显高于正常组(P<0.05),PYAP蛋白表达明显低于正常组(P<0.05)。结论 细胞脓毒症模型中YAP的高表达及PYAP的低表达可能参与了脓毒症的发生。

    Abstract:

    【Abstract】 Objective To investigate the expression of YAP and PYAP in sepsis model of RAW2647 cells. Methods RAW2647 cells were stimulated by lipopolysaccharide (LPS) to establish the model of sepsis。The location of YAP and PYAP was detected by immunofluorescence, and the expressions of YAP and PYAP were detected by Western blot. Results In the sepsis model of RAW2647 cells, the YAP protein was expressed in the nucleus, whereas the PYAP was expressed in the cytoplasm. In the model of sepsis, the expression of YAP protein was significantly higher than that in the normal group, and the expression of PYAP protein in the sepsis model was lower than that in the normal group. Conclusion High expression of YAP and low expression of PYAP may be involved in the development of sepsis in sepsis model.

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  • 在线发布日期: 2018-04-18
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