Abstract:【Abstract】 Objective To investigate the expression of YAP and PYAP in sepsis model of RAW2647 cells. Methods RAW2647 cells were stimulated by lipopolysaccharide (LPS) to establish the model of sepsis。The location of YAP and PYAP was detected by immunofluorescence, and the expressions of YAP and PYAP were detected by Western blot. Results In the sepsis model of RAW2647 cells, the YAP protein was expressed in the nucleus, whereas the PYAP was expressed in the cytoplasm. In the model of sepsis, the expression of YAP protein was significantly higher than that in the normal group, and the expression of PYAP protein in the sepsis model was lower than that in the normal group. Conclusion High expression of YAP and low expression of PYAP may be involved in the development of sepsis in sepsis model.