Abstract:【Abstract】 Objective To observe the histological changes of tendon graft pretreated by deep frozen and irreversible electroporation, and assess the feasibility of treatment for tendon defect by using irreversible electroporationpretreated tendon allograft. Methods 112 New Zealand white rabbits were randomly divided into autologous transplantation group, frozen tendon allograft transplantation group, irreversible electroporation ablated tendon allograft transplantation group, and sham operation control group, 28 in each group. The deep flexor tendon defect of the third toe was prepared and the corresponding defect was repaired by treated tendon. Specimen were collected at 1 week, 4 weeks, 8 weeks and 12 weeks post operation for histologic observation, quantitative analysis of microvessel regeneration and biomechanical evaluation.Results There were no significant differences in the tendon diameter between the four groups at each time point (P > 005). Histological observation showed that fibroblast replacement and microvascular regeneration in group B were lower than that in group A and group C. Microvascular perfusion showed that effective perfusion areas in group A, B, C were significantly lower than that in group D at 1 week (P < 005), and effective perfusion areas in group B was significantly lower than that in group A, C, D from 4 week to 12 week (P < 005). At the 12th week, group B was significantly lower than the group A, C & D, the difference was statistically significant (P <005). The biomechanical results showed that there was significant difference in the maximal load and stiffness of tendon between group B and group D at 4 weeks after operation (P < 005). There was no significant difference in damage deformation between the four groups at each time point (P > 005). Conclusion Irreversible electroporation ablated tendon allograft can be used to repair tendon defect, and the morphological and biomechanics property of tendon ablated by irreversible electroporation has no significant difference with autologous transplantation group.