【Abstract】〓Objective〓To analyze the distribution of clinical pathogenic bacteria and it's drug resistance and provide laboratory evidence for clinical treatment．Methods〓 Based on the technique of 2014CLSI,the WHONET 56 was used to analyze the data of the distribution and drug resistance of all clinical isolates from the hospital during this 5 years.Results〓A total of 8645 strains bacteria were isolated, including 6886（693%）Gramnegative bacteria and 1759（307%）Grampositive bacteria strains. The top 10 bacteria were E. coli, Klebsiella pneumonia, Staphylococcus aureus, Pseudomonas aeruginosa, Haemophilus influenza, Streptococcus pneumonia, Acinetobacter baumannii, Enterobacter cloacae, Moraxella catarrhalis, Proteus mirabilis. In Grampositive bacteria, Staphylococcus aureus (MRSA) detection rates were 174%. In Enterobacteriaceae, E. coli and klebsiella pneumonia bacteria (ESBLs) rate were 445% and 197% respectively. The rate of E. coli and klebsiella pneumonia with Carbapenemsresistant were 04% and 09% respectively, but 186% Pseudomonas aeruginosa and 252% Acinetobacter baumannii were found. There was no E faecium and E faecalis which was resistant to vancomycin.Conclusion〓〓It has great significance to investigate and analyze the pathogenic bacteria with their distribution and drug resistance for clinical treatment and reduction of drug resistant.