【Abstract】Objective To investigate the effect of ulinastatin combined with octreotide on cytokines in patients with acute pancreatitis. Methods 126 patients with acute pancreatitis admitted to our hospital from March 2012 to July 2016 were randomly divided into octreotide group, ulinastatin group and combined group. Each group consisted of 42 patients. The patients in the three groups were treated with routine therapy. The octreotide group was given intravenous injection of octreotide. The ulinastatin group was treated with ulinastatin. The combination group was treated with the combination of octreotide and ulinastatin. The clinical data of 3 groups were retrospectively analyzed, and the clinical curative effect and cytokine effect were analyzed. Results In octreotide group, 13 cases were cured, 14 cases were effective and 15 cases were ineffective. The total effective rate of octreotide group was 6429%. In ulinastatin group, 14 cases were cured, 12 cases were effective and 16 cases were ineffective. The total effective rate of ulinastatin group was 6190%. In combination group, 28 cases were cured, 11 cases were effective and 3 cases were ineffective. The total effective rate of combination group was 9286%. The total effective rate the three groups were obviously difference (P＜0.05). The cytokines in the three groups before treatment were not significantly different (P＞0.05). The cytokines in the three groups after treatment were significantly different (P＜0.05). The complication rates of octreotide group, ulinastatin group and combined group were 1111%, 793% and 3.17%. The complication rates of octreotide group and ulinastatin group were statistically different from that of combined group (P＜0.05). Conclusion Ulinastatin combined with octreotide in the treatment of acute pancreatitis is effective in the treatment of acute pancreatitis. It can significantly improve the clinical signs and symptoms, reduce the incidence of complications and improve the safety of the treatment.