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传染性单核细胞增多症患儿外周血单个核细胞EB病毒负荷的荧光定量PCR检测及临床价值
王惠霞,袁粒星,高举
0
(西北妇女儿童医院儿科;四川大学华西第二医院,儿童公共实验室;四川大学华西第二医院儿童血液肿瘤科)
摘要:
【摘要】目的 研究传染性单核细胞增多症(IM)患儿外周血单个核细胞(PBMC)EBVDNA负荷及其与临床特征的相关性。方法 实验组为华西第二医院收治的80例IM患儿临床诊断病例,对照组为40例健康儿童,均采用荧光定量PCR方法检测研究对象PBMC EBVBALF5基因拷贝数,分析其与ALT、AST、LDH、WBC和变异淋巴细胞比例的相关性。结果 实验组80例患儿中73例检测出EBVBALF5基因(91.25%),拷贝数介于103~105 copies/ml,中位数约2.4×10.4 copies/ml。对照组40例儿童中2例检出(500%),结果表明IM患儿EBVBALF5拷贝数显著高于对照组(P<0.001)。以拷贝数1×10.4copies/ml为界,又将实验组分为较高拷贝组(拷贝数≥1×104copies/ml)和低拷贝组(拷贝数<1×10.4copies/ml),发现较高拷贝组ALT和AST升高的概率高于低拷贝组(P<0.05)。ALT、AST、LDH与EBVBALF5拷贝数呈正相关关系(P<0.05)。结论 采用荧光定量PCR可有效检测出IM患儿PBMC EBVBALF5, EBVBALF5拷贝数与IM患儿肝功损害的发生相关,拷贝数越高,ALT、AST和LDH 等肝功酶学指标升高更明显。
关键词:  传染性单核细胞增多症  EB病毒  荧光定量PCR-1
DOI:
基金项目:
Measurement of Epstein Barr virus DNA load in peripheral blood mononuclear cells by absolute fluorescent quantitative PCR in children with infectious mononucleosis and possible implications
WANG Huixia,YUAN Lixing,GAO Ju
(Department of Pediatrics, Northwest Women and Children Hospita;Child Public Laboratory,West China Secand Hospital,Sichuan Clniversity;Depantment of Hematologic neoplasms of Child,Sichuan Clniversity,West China Second Hospita)
Abstract:
【Abstract】Objectives The aims of this research were to quantitatively measure copy numbers of EBVBALF5 gene in peripheral blood mononuclear cells from children with infectious mononucleosis and to correlate copy numbers with clinical features.Methods 80 children with newly diagnosed infectious mononucleosis and 40 healthy control children were enrolled in this study. EBVBALF5 copy numbers in mononuclear cells from IM patients and control children were determined by absolute fluorescence quantitative PCR and correlate copy numbers with ALT, AST, LDH, WBC and percentage of atypical lymphocytes. Results EBVBALF5 gene was detected in 73 children with IM, with copy numbers ranging from 10.3~10.5 copies/ml (median copy number 2.4(10.4copies/ml).EBVBALF5 gene was also amplified in 2 control children, but with much lower copy numbers. Rank correlation test revealed that copy numbers in IM group were statistically higher than that in control children. Samples were divided into high copy number group(arbitrarily defined as copy numbers(1(10.4copies/ml) and low copy number group(arbitrarily defined as copy number<1(10.4copies/ml).The probability of liver function abnormality in higher copy number group was statistically higher than that in low copy number group(P<0.05). Ranked correlation test revealed that EBVBALF5 copy numbers were positively correlated with increment of ALT, AST and LDH, but were not correlated with total WBC count and percentage of atypical lymphocytes in the peripheral blood. Conclusions EBVBALF5 gene load could be reliably measured by absolute fluorescence quantitative PCR. Copy number of EBVBALF5 gene is correlated to the likelihood of organ function damage such as liver enzyme increase.
Key words:  Infectious mononucleosis  Epstein Barr virus  Fluorescent quantitative PCR

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