【Abstract】Objective To investigate the P16, ki67 and Bcl2 value in cervical adenocarcinoma in situ pathological diagnosis. Methods Thirty patients of cervical paraffin sections were collected from cervical adenocarcinoma (group A), cervical adenocarcinoma (group B) and normal cervical tissue (group C). The antibody P16, ki67 and Bcl were detected by SP immunohistochemistry2 to express the situation, and based on statistical results to understand the three antibodies combined detection of cervical in situ adenocarcinoma specificity and sensitivity. Results P16 with Ki67 expression in group A and group B increased compared that in group C (P<0.05). Bcl2 of group A and group B were significantly higher than that of group C (P<0.05). The sensitivity of P16, Ki67 and Bcl2 in diagnosis of cervical adenocarcinoma was 90%, 50% and 13.33%, respectively, and the specificity was 62.07%, 72.97% and 52.63%, respectively. The sensitivity and specificity of diagnosis of cervical adenocarcinoma were 11.23% and 37.92%, respectively. The sensitivity (63.33%) and specificity (69.44%) of positive P16 and Ki67 were higher than those of negative P16 and B16 Ki67 (P<0.05). Conclusion The clinical diagnosis of cervical adenocarcinoma is often nonspecific. The pathological diagnosis needs immunohistochemistry. When immunohistochemistry suggests that positive P16 and negative Ki67, Bcl2 can improve the cervical adenocarcinoma diagnostic specificity and sensitivity.