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Nrf-2与HO-1在胃癌组织中的表达及意义
蒋龙超,胡双丽,唐世孝,武伟
0
(泸州市人民医院;西南医科大学附属医院)
摘要:
【摘要】目的 通过检测核因子E2相关因子(Nrf-2)、血红素加氧酶1(HO-1)在胃粘膜标本组织中的表达情况,研究二者在胃癌发生、发展以及早期胃癌诊断中的意义和作用。方法 通过免疫组化EnVision法检测122例Nrf-2、HO-1在胃粘膜标本组织中的表达情况,分析 Nrf-2、HO-1在胃粘膜组织各个病变期彼此间的关系,以及二者与胃癌各临床病理资料因子相应的关系。结果 Nrf-2蛋白在正常胃粘膜组与重度不典型增生组、胃癌组对照比较,差异有统计学意义(P<0.05);肠上皮化生组与胃癌组对照比较,差异有统计学意义(P<0.05);轻中度不典型增生组与胃癌组对照比较,差异有统计学意义(P<0.05);而其余各胃粘膜样本组之间相互对照比较,差异无统计学意义(P<0.05)。HO-1蛋白在正常胃粘膜组与重度不典型增生组、胃癌组对照比较,差异有统计学意义(P<0.05);肠上皮化生组与胃癌组对照比较,差异有统计学意义(P<0.05);轻中度不典型增生组与胃癌组对照比较,差异有统计学意义(P<0.05);而其余各胃粘膜样本组之间相互对照比较,差异无统计学意义(P〉0.05)。Nrf2、HO-1蛋白分别在年龄、性别、肿瘤大小、分化程度中比较,差异无统计学意义(P〉0.05);在肿瘤侵润深度、TNM分期、淋巴结转移中比较,差异有统计学意义(P<0.05)。结论 Nrf-2、HO-1的高表达对胃癌的发生、发展可能有重要作用,若将二者进行联合检测,对胃癌高危人群的初筛、早期胃癌的诊断和预后的判断可能有重要的意义。同时二者也将有可能成为胃癌化学预防以及治疗的新靶点。
关键词:  Nrf-2  HO-1  胃癌前病变  胃癌  免疫组织化学
DOI:
基金项目:泸州市科技计划项目(2015LZCYD S01 9/15)
Expression and significance of Nrf-2 and HO-1 in gastric cancer
JIANG Longchao,HU Shuangli,TANG Shixiao,WU Wei
(Luzhou People's Hospital;Department of Pharmacy, The Second People's Hospital of Yibin)
Abstract:
【Abstract】Objective To detect the expression levels of nuclear factor erythroid-2related factor2 and heme oxygenase-1 in all sample tissues, study the significances of Nrf-2 and HO-1 in the development and the early diagnosis of gastric cancer and investigate the relevance between Nrf-2 and HO-1 and the mechanism in the process of gastric mucosal canceration. Methods Immunohistochemistry EnVisionmethod was used to detect the expression of Nrf-2 and HO-1 in each group and the relationship between Nrf-2 and HO-1 in various lesions of the gastric mucosa. The correlation with clinical pathological characteristics of gastric cancer were analyzed. Results The differences between normal group and severe atypical hyperplasia group (P=0.014), gastric cancer group (P=0.000) were statistically significance which the positive expression of Nrf-2. The differences between intestinal metaplasia group and gastric cancer group(P=0.006) were statistically significance which the positive expression of Nrf-2. The differences between mildmoderate atypical hyperplasia group and gastric cancer group(P=0.004) were statistically significance which the positive expression of Nrf-2. The differences between the rest groups were not statistically significance. The differences between normal group and severe atypical hyperplasia group(P=0.002),gastric cancer group(P=0.000) were statistically significance which the positive expression of HO-1. The differences between intestinal metaplasia group and gastric cancer group(P=0.016) were statistically significance which the positive expression of HO-1. The differences between mildmoderate atypical hyperplasia group and gastric cancer group(P=0.009) were statistically significance which the positive expression of HO-1. The differences between the rest groups were not statistically significance. The differences of Nrf-2 and HO-1 were not statistically significance in different age, gender, tumor size and degree of tumor differentiation(P>0.05). Tthe differences were statistically significance in different depth of invasion of the gastric wall, TNM stage, lymph node metastasis (P<0.05). Conclusion This experiment suggesting that both Nrf-2 and HO-1 may play an important role in occurrence and development of gastric cancer. Joint detection of the two proteins may have important significance for gastric cancer screening of highrisk population, early diagnosis of gastric cancer and judgment of prognosis.
Key words:  Nrf-2  HO-1  Gastric precancerous lesions  Gastric cancer  Immunohistochemistry

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