引用本文:[点击复制]
[点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 130次   下载 9 本文二维码信息
码上扫一扫!
Tat蛋白对节律基因表达的影响
丁璐,应俊杰,王正荣,后望,江舟,刘延友,汪宇辉,成姝婷,肖静
0
(四川大学华西基础医学与法医学院·卫生部时间生物学重点实验室)
摘要:
【摘要】目的 探讨HIV Tat蛋白是否可以在细胞内影响主要节律基因Clock,Cry,Bmal1,Per的表达。方法 用lipofectamineTM2000将HIVat表达质粒转入PC12细胞,使Tat在PC12细胞中表达。转染48h后提取mRNA和蛋白,分别通过实时荧光定量PCR技术和Westernblot技术检测节律基因在mRNA和蛋白水平表达的变化。结果 研究结果显示,与未处理组和空质粒组相比,转染组Cry1的表达水平显著升高(P<0.05);Clock的表达水平显著降低(P<0.05);Bmal1和Per的表达水平无明显变化(P>0.05)。 结论 HIV Tat影响了近日节律基因Cry1和Clock的表达,表现为Cry1表达水平升高,Clock表达水平下降,提示TAT蛋白可以影响节律基因的表达并且可能通过对近日节律的影响调节细胞生理功能。
关键词:  Tat蛋白  节律基因  PC 12细胞
DOI:
基金项目:国家自然科学基金(31371180);四川省科技支撑计划项目(2014SZ0193)
Effect of Tat on crrcadian genes
DING Lu,YING Junjie,WANG Zhengrong,HOU Wang,JIANG Zhou,LIU Yanyou,WANG Yuhui,CHENG Shuting,XIAO Jing
(Key Laboratory of Chronobiology,Ministry of Health (Sichuan University), West China School of Preclinical and Forensic Medicine, Sichuan University)
Abstract:
【Abstract】Objective To explore whether HIV TATprotein can affect the expression of Clock, Cry,Bmal1,Per in the cell. Methods HIVTat protein expression plasmids were transfected into PC12 with lipofectamine TM 2000, and Tat was expressed in PC12.mRNA and protein were extracted 48 hours after transfection, and the expression of rhythm gene was detected by realtime quantitative PCR and Western blot, respectively.Results The results showed that the expression level of Cry1was significantly increased(P<0.05). The expression ofClock was significantly decreased(P<0.05). The expression of Bmal1and Perwas not significantly changed in transfected Tatexpressing plasmid compared with untransfected and empty plasmid. Conclusion HIV Tat affects the expression of the circadian genes Cry1 and Clock, which is related to the decrease ofClock expression and the increase of Cry1 expression, Suggesting that TAT protein can affect the expression of rhythmic genes and may regulate the physiological function of cells through the influence of circadian rhythm.
Key words:  Tat protein  Circadian gene  PC 12 cell

用微信扫一扫

用微信扫一扫