【Abstract】 Objective We record and analysis connexin 26(Cx26) protein expression in different types of gastric mucosa, study the expression, change rule, and mechanism in types of cell and discuss the clinical value of Cx26 in the early diagnosis of gastric cancer. Methods We detected the expression of Cx26 by Immunohistochemical Envision method of 169 samples, analyzed the relationship in each groups, evaluated and analyzed the result by the standard Shimizu scoring method. Results Cx26 expression level increased while diffrentiation level declined. Atypical hyperplasia and normal gastric mucosa tissues Cx26 protein positive expression levels were far below the expression level in gastric cancer tissue (p＜005),and it related to tumor types. Positive expression rate of intestinal gastric cancer was significantly higher than that of diffuse gastric cancer(p＜005). Conclusion Cx26 inhibited progression and metastasis of gastric cancer, which maybe related to its regulation of cell communication，and promoting cell differentiation.