【Abstract】 Objective To analyze the expressions of TrkB and GLT-1 in the brain of pentylenetetrazol (PTZ) kindling model of chronic epilepsy in rats, and provide evidence for correlation between the BDNF/TrkB pathway and GLT-1 and clues for the prevention and treatment targets of epilepsy.Methods 40 SpragueDawley (SD) rats were randomly classified as model (30) and control (10) groups, respectively. Western blotting was used for analyzing the expression of TrkB and GLT-1, and double immunofluorescence staining was used for detecting the cells positive for both glial fibrillary acidic protein (GFAP) and TrkB in the hippocampus and temporal cortex 7 days after establishing the kindling modelResults Compared with the control group, protein level expression of TrkB and GLT-1, and mean optical density (OD) value of the cells positive for both GFAP and TrkB in the hippocampus and temporal cortex were significantly increased after the onset of epilepsy (P<0.05). Conclusion The BDNF/TrkB pathway may participate in epileptogenesis through modulating the biological effect of GLT-1 in the glial cells of PTZ kindling rats, though the specific mechanism requires further investigation.