【Abstract】 Objective To investigate the clinical values of 12 lead dynamic electrocardiogram in evaluating myocardial bridge in patients with myocardial ischemia. Methods From February 2013 to January 2016, 98 patients with coronary myocardial bridge confirmed by coronary arteriography were selected in Shunyi hospital of Beijing Chinese medicine hospital. All patients were given the 12 lead dynamic electrocardiogram detecting, recorded and observed the myocardial ischemia, myocardial bridge and prognosis. Results In the 98 cases, the diagnosis of coronary angiography were 40 cases of single vessel disease (single branch group) and 58 cases with multiple lesions (multi branch group). There were 87 patients with myocardial ischemia for predicting by the 12 lead dynamic electrocardiogram. The myocardial ischemia detection rate was 888%. The detection rate, total duration and ST segment depression range of the multi branch group were significantly higher than the single branch group (P＜005). 12 lead dynamic electrocardiogram judgment for 70 cases were superficial type. The deep type were 28 cases. The length of myocardial bridge were (1834±419)mm, and the stenosis degree of myocardial bridge were (5813±1174)%. The length of myocardial bridge and stenosis degree of myocardial bridge in the multi branch group compared to the single branch lesion group had statistical differences significance (P＜005). All patients were followed up after discharge, and there were 8 cases of major adverse cardiac events. The incidence rate was 82%, while the branch group of adverse cardiac events was significantly higher than that of single vessel disease group (P＜005). Conclusion 12 lead dynamic electrocardiogram can be more intuitive accurate evaluation of myocardial ischemia in patients. The changes of ST segment of dynamic electrocardiogram is more significant in patients with multiple lesions, so as to guide the prediction prognosis of adverse cardiac events and also with noninvasive, high safety and other advantages.